Molecular Biomarkers & Diagnosis

Juxian Lu-Hesselmann

Publications

• Research Article
  Detection of Free Radical Reaction Products and Activated Signalling Molecules as Biomarkers of Cell Damage in Human Keratinocytes upon Lead Exposure
  Author(s): Birgit Bölck, Marwa Ibrahim, Juxian Lu-Hesselmann, Dirk Steinritz, Frank Suhr and Wilhelm BlochBirgit Bölck, Marwa Ibrahim, Juxian Lu-Hesselmann, Dirk Steinritz, Frank Suhr and Wilhelm Bloch
  
  Lead (Pb) is one of the most important environmental pollutant metals accumulating in the atmosphere, water, foods, and in organisms living in contaminated areas. Skin is one of the main targets of Pb toxicity based on its ability of direct penetration upon exposure. The underlying cell damaging pathomechanisms have not been revealed in detail. Herein, we focus on Pb-induced oxidative and nitrosative stress that has not been previously thoroughly investigated. We investigated these effects in order to elucidate the pathomechanisms and as well to identify potential biological markers that may indicate Pb toxicity. Human immortalized keratinocytes (HaCaT cells) were exposed to Pb (100 μM) either for 5 minutes or 6 hours. Pb-induced cellular damage was evaluated by immunocytochemistry analysis of multiple signalling cascades, e.g. apoptosis, Akt, MAPK, NOS, nitrotyrosine and 8-isopros.. Read More»
  DOI: 10.4172/2155-9929.1000179

  Abstract PDF