Environmental & Analytical Toxicology

KA McVey

Publications

• Research Article
  Organophosphorous Pesticides Metabolite Reduces Human T CD8 Homeostasis and Proliferation by Inducing Cellular Death
  Author(s): KA McVey, JA Mink, IB Snapp, CE Todt, R Negga and VA FitsanakisKA McVey, JA Mink, IB Snapp, CE Todt, R Negga and VA Fitsanakis
  
  Background: We have previously shown that Diethyldithiophosphate (DEDTP), a metabolite of Organophosphorous (OP) compounds biotransformation with longer half life than its parental compound, can modulate T CD4 lymphocyte functions. To explore if DEDTP can also alter T CD8 homeostasis and proliferation we evaluated cellular viability and proliferation by propidium iodide (PI) incorporation and carboxyfluorescein succinimidyl ester (CFSE) assay by flow cytometry, respectively, in peripheral blood mononuclear cells (PBMCs) and T CD8 cells from healthy male donors. Results: In vitro exposure to 1-50 μM DEDTP decreased T CD4 vs. T CD8 proportion on resting T CD3 lymphocytes. DEDTP decreased T CD8 viability in a dose-dependent manner after 24 h without affecting the rest of T CD3 lymphocytes. DEDTP also decreases CFSE dilution in T CD8 cells stim.. Read More»
  DOI: 10.4172/2161-0525.S4-004

  Abstract PDF