Journal of Tissue Science and Engineering

Kancherla Ravindranath

Publications

• Research Article
  In Vitro Development of an Acellular Biological Liver Scaffold Repopulated with Rat Hepatocytes
  Author(s): Tanya Debnath, Kancherla Ravindranath, Chandra Shekar Mallarpu, Pavan Mujawdiya, Suman Kapur and Lakshmi Kiran ChelluriTanya Debnath, Kancherla Ravindranath, Chandra Shekar Mallarpu, Pavan Mujawdiya, Suman Kapur and Lakshmi Kiran Chelluri
  
  The increasing demand of organs for transplantation necessitates the development of substitutes to meet the structural and physiological functions. Tissue decellularization and recellularization aids in retaining the three-dimensional integrity, biochemical composition, tissue ultra-structure, and mechanical behavior, which makes them functionally suitable for organ transplantation. Herein, we attempted to rebuild functional liver grafts in small animal model (Wistar rat) with a potential of translation. A soft approach was adopted using 0.1% SDS (Sodium Dodecyl Sulfate) for decellularization and primary hepatocytes were used as potential cell source for recellularization. The decellularization process was evaluated and confirmed using histology, DNA content, ultra-structure analysis. The resultant scaffold was re-seeded with the rat hepatocytes and their biocompatibility was assessed.. Read More»
  DOI: 10.37421/jtse.2019.10.224

  Abstract PDF