India
Research Article
A Comparative Study of New Castle Disease Virus Isolated From Poultry with Standard Velogenic Strain
Author(s): Karmakar DB, Majee SB, Karikkathil SS, Seth D and Mondal SKarmakar DB, Majee SB, Karikkathil SS, Seth D and Mondal S
Newcastle disease [avian paramyxovirus serotype 1 (APMV-1)] is highly prevalent in poultry thought India. The present study was undertaken to extrapolate the pathogenecity of a field isolate of Newcastle disease virus (NDV) in comparison with velogenic BOM-1 isolate (AY 294125). The field NDV isolate and the BOM-1 isolate were propagated in SPF eggs and the infected allantoic fluids were used for characterization of pathotype. The Mean Death Time (46 hours), Intracerebral Pathogenicity Index (1.74), Intravenous pathogenicity Index (2.01) were enumerated to find out the pathotype of the virus. The field NDV isolate was able to agglutinate mammalian erythroytes except equine RBCs, stable at 56°C for 60 mins and proved to be a rapid eluter. It formed plaques (5.39 x 106 PFU/ ml) in chick embryo fibroblast with characteristic cytopathic effects of velogen.. Read More»
DOI:
10.4172/2157-7579.1000231
Veterinary Science & Technology received 4472 citations as per Google Scholar report