Journal of Cytology & Histology

Karl-Henrik Grinnemo

Publications

• Research Article
  Human Embryonic Non-haematopoietic SSEA-1+ Cells are Cardiac Progenitors Expressing Markers of Both the First and Second Heart Field
  Author(s): Elzafir Elsheikh, Rami Genead, Agneta Mansson-Broberg, Ivana Bulatovic, Karin Ljung, Eva Wärdell, Ulrika Felldin, Cristián Ibarra, Evren Alici, Christer Sylvén and Karl-Henrik GrinnemoElzafir Elsheikh, Rami Genead, Agneta Mansson-Broberg, Ivana Bulatovic, Karin Ljung, Eva Wärdell, Ulrika Felldin, Cristián Ibarra, Evren Alici, Christer Sylvén and Karl-Henrik Grinnemo
  
  Background: Damage to the myocardium following a myocardial infarction can be severe and possibly non reversible leading to heart failure. However, recent evidence suggests that cardiomyocyte progenitor cells may be able to assist in healing the injured cells. The study purpose is to investigate the use of “Stage Specific Embryonic Antigen 1” (SSEA-1) surface marker to isolate cardiac progenitors from the human embryonic heart. Materials and Methods: The surface marker SSEA-1 was used to isolate cardiac progenitor cells from human embryonic hearts obtained from abortion material. The fast and reliable Fluorescence Activated Cell Sorting (FACS) technique was used to sort out pure population of SSEA-1+ cells. Cells were then cultured and characterized for multipotent stem cells markers using qRT-PCR and flow cytometry. The car.. Read More»
  DOI: 10.4172/2157-7099.1000192

  Abstract PDF