Biosensors & Bioelectronics

Kavita Arora

Publications

• Research Article
  Graphene Oxide based Label Free Ultrasensitive Immunosensor for Lung Cancer Biomarker, hTERT
  Author(s): Meenakshi Choudhary, Veeresh Kumar, Anu Singh, Manoj P. Singh, Satbir Kaur, G.B. Reddy, Renu Pasricha, Surinder P. Singh and Kavita AroraMeenakshi Choudhary, Veeresh Kumar, Anu Singh, Manoj P. Singh, Satbir Kaur, G.B. Reddy, Renu Pasricha, Surinder P. Singh and Kavita Arora
  
  We report the fabrication of ultrasensitive Graphene Oxide (GO) based electrochemical immunosensor to detect human telomerase reverse transcriptase (hTERT), a lung cancer biomarker. The immuno-electrode-has been fabricated by covalent immobilization of rabbit anti-hTERT antibodies (Ab) onto GO films on ITO coated glass. The Fourier Transform Infrared (FTIR) spectroscopic studies confirms the presence of diverse organic functional groups (-COOH, -CHO, -OH) of GO, and the binding (anti-hTERT) onto GO/ITO electrode. Interestingly, Scanning Electron Micrographs (SEM) also reveals clear visual surface modification of the GO film by anti-hTERT antibodies and hTERT antigen (Ag). The electrochemical Differential Pulse Voltammetry (DPV) results show that the GO based immunosensor exhibits specificity and low detection upto 10 ag mL-1 (10×10-18 g mL-1) in wide detection range (10 ag mL-1-5.. Read More»
  DOI: 10.4172/2155-6210.1000143

  Abstract PDF