Journal of Health & Medical Informatics

Kazuo Maeda

Publications

• Research Article
  Human Embryonic Behavior Observed with Time-Lapse Cinematography
  Author(s): Yasuyuki Mio, Kyoko Iwata, Keitaro Yumoto and Kazuo MaedaYasuyuki Mio, Kyoko Iwata, Keitaro Yumoto and Kazuo Maeda
  
  To clarify developmental behavior in human embryos, we observed oocytes with in vitro fertilization and intracytoplasmic sperm injection and donated embryos up to 5 days using our original time-lapse cinematography (TLC) system. The TLC analyses demonstrated several novel aspects and events that were not visible by intermittent stillframe microscopy. Here, we present the entire fertilization process in which we founded the fertilization cone, cytoplasmic flare, dynamic change of nuclear precursor body and cytoplasmic halo. We also demonstrated the occurrence of zygotes with a single pronucleus (PN) and uneven 2PN, fragmentation, blastocoel collapse during the blastocyst stage, and the hatching pattern of expanded blastocysts. Observing such morphokineses in human embryos will enhance our understanding of human embryonic behavior up to preimplantation... Read More»
  DOI: 10.4172/2157-7420.1000143

  Abstract PDF