Journal of Clinical Case Reports

Keiryn L Bennett

Publications

• Case Report
  Proteomic and Clinical Analysis of a Fine-Needle Aspirate Biopsy from a Single Cold Thyroid Nodule: A Case Study
  Author(s): Dijana Vitko, Fernando J. Sialana, Katja Parapatics, Oskar Koperek, Christian Pötzi, Shuren Li and Keiryn L BennettDijana Vitko, Fernando J. Sialana, Katja Parapatics, Oskar Koperek, Christian Pötzi, Shuren Li and Keiryn L Bennett
  
  Background: For cases where clinical and cytological data from cold thyroid nodules are ambiguous; presurgical proteomic profiling of fine-needle aspirate biopsies of cold thyroid nodules in situ can provide additional diagnostics to avoid invasive surgical intervention and thyroidectomy of benign or non-cancerous tissue. Methods: The fine-needle aspirate biopsy lysate was digested with trypsin, and analysed by liquid chromatography mass spectrometry on a linear trap quadrupole Orbitrap Velos. Remaining peptides were separated by reversedphase chromatography and fractions analysed as technical duplicates. Identified proteins were analysed by Gene Ontology and protein abundance were calculated using the Top3 label-free method. The proteomic data was complemented with ultrasonography and scintigraphy of the thyroid gland; and cytology of the cold thyroi.. Read More»
  DOI: 10.4172/2165-7920.1000766

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