Biosensors & Bioelectronics

Kentaro Ono

Publications

• Research Article
  BSA Adsorption and Immobilization onto Charged Monodisperse Polymer Nanoparticles
  Author(s): Masahiro Yasuda, Kentaro Ono, Takashi Nomura, Scott H Brewer, Oyvind Halskau Jr, Sondre Volden and Wilhelm R GlommMasahiro Yasuda, Kentaro Ono, Takashi Nomura, Scott H Brewer, Oyvind Halskau Jr, Sondre Volden and Wilhelm R Glomm
  
  Controlling the behavior of adsorbed and immobilized proteins is essential for protein purification and a wide range of applications, including biosensors, biocatalysis and biomedical devices. In this study, monodisperse polymer particles were synthesized by soap free emulsion polymerization and the surface functional groups were directly introduced as a monomer or chemically modified with epoxy groups to enable protein immobilization through covalent bonding. Three kinds of surface charged particles having cationic, anionic, or both, groups were synthesized and characterized. Bovine serum albumin (BSA) was selected as a model protein to study the effect of pH of a buffer solution containing protein on the adsorption and immobilization amount. Protein adsorption was found to be strongly affected by pH and matching of the global protein and polymer particle charges, respectively. When .. Read More»
  DOI: 10.4172/2155-6210.1000183

  Abstract PDF