Hydrology: Current Research

Khwam R. Hussein

Publications

• Research Article
  Development and use of Bacteroides 16S rRNA Polymerase Chain Reaction Assay for Source Tracking Dog Faecal Pollution in Bathing Waters
  Author(s): Khwam R. Hussein, Paul L. Waines, Raid B. Nisr, Gillian Glegg and Graham BradleyKhwam R. Hussein, Paul L. Waines, Raid B. Nisr, Gillian Glegg and Graham Bradley
  
  Faecal pollution on bathing beaches poses a potential threat to human health and as a result may also negatively affect the local economy. In instances where the source of such pollution is not obvious, it may be necessary to track such sources using a host-specific genetic markers technique. Bacteroides species are potential indicators for source tracking of faecal pollution in bathing waters. This study designed specific primer sets to amplify sections of the 16S rRNA gene unique to Bacteroides from domestic dogs and used quantitative PCR (qPCR) to quantify such genetic markers in environmental samples. The sensitivity and specificity of the primer sets was determined; they were specific in silico against known dog Bacteroides sequences and in vitro against Bacteroides sequences originating from human and livestock faeces. Dog faecal Bacteroides contamination was then detected in se.. Read More»
  DOI: 10.4172/2157-7587.1000163

  Abstract PDF