Journal of Bioanalysis & Biomedicine

Kristine E. Mulier

Publications

• Research Article
  Urine Metabolomics in Hemorrhagic Shock: Normalization of Urine in the Face of Changing Intravascular Fluid Volume and Perturbations in Metabolism
  Author(s): Elizabeth R. Lusczek, Teresa Nelson, Daniel Lexcen, Nancy E. Witowski, Kristine E. Mulier and Greg BeilmanElizabeth R. Lusczek, Teresa Nelson, Daniel Lexcen, Nancy E. Witowski, Kristine E. Mulier and Greg Beilman
  
  There are many ways to normalize biofluid metabolomics data to account for changes in dilution, all of which have been thoroughly examined in model systems. Here, urine metabolomics data was examined under relevant physiological conditions obtained from a porcine model of hemorrhagic shock and resuscitation. This includes highly variable intravascular fluid volume and urine output coupled with large perturbations in the abundance of endogenous metabolites. Seven different normalization techniques and raw data were evaluated to determine an appropriate normalization technique in this setting, including spectral post-processing methods and physiological measures of concentration. Relationships between normalization constants for each urine sample were examined, as well as relationships between urinary and serum creatinine concentrations. Principal components analysis was used to examine.. Read More»
  DOI: 10.4172/1948-593X.1000041

  Abstract PDF