Journal of Tissue Science and Engineering

Kum Fai Chooi

Publications

• Research Article
  Improvement of Gene Expression Studies in the Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat Using Selected Reference Genes for Quantitative Real Time-PCR Analysis
  Author(s): Dinesh Babu Kuppan Rajendran, Gary Phang Siew Siang, Alden Toh Han Hui and Kum Fai ChooiDinesh Babu Kuppan Rajendran, Gary Phang Siew Siang, Alden Toh Han Hui and Kum Fai Chooi
  
  Background: Liver fibrosis is a reaction to chronic liver injury characterized by excessive accumulation of collagen. Due to their importance as biomarkers, the changes in gene expression in the liver during the development of fibrosis and its subsequent outcomes of cirrhosis, neoplasia or resolution are intensely studied. Quantitative realtime PCR (qPCR) with its ability to detect and measure minute amounts of nucleic acids have been increasingly used in these studies. In qPCR, the quantitation of mRNA is relative and the accuracy of results dependent on the reference genes used for standardization. However, many genes studied are normalized against single reference genes, usually housekeeping genes, without adequate justification. Methods: For the dimethylnitrosamine (DMN) induced liver fibrosis rat model, we tested 8 commonly used candidate .. Read More»
  DOI: 10.4172/2157-7552.1000183

  Abstract PDF