Molecular Biomarkers & Diagnosis

Lars Dyrskjøt

Publications

• Research Article
  TOX3 (TNRC9) Over Expression in Bladder Cancer Cells Decreases Cellular Proliferation and Triggers an Interferon-Like Response
  Author(s): Karin Birkenkamp-Demtroder, Francisco Mansilla, Lars Dyrskjøt, Kasper Thorsen, Niels Fristrup, Anne Sofie Brems-Eskildsen, Pia Pinholt Munksgaard, Karina Dalsgaard Sørensen, Michael Borre and Torben Falck ØrntoftKarin Birkenkamp-Demtroder, Francisco Mansilla, Lars Dyrskjøt, Kasper Thorsen, Niels Fristrup, Anne Sofie Brems-Eskildsen, Pia Pinholt Munksgaard, Karina Dalsgaard Sørensen, Michael Borre and Torben Falck Ørntoft
  
  Background: Human TOX3 (TOX high mobility group box family member 3) regulates Ca2+ dependent transcription in neurons and has been associated with breast cancer susceptibility. Aim of the study was to investigate the expression of TOX3 in bladder cancer tissue samples and to identify genes and pathways altered upon TOX3 dysregulation using a cell line model. Methods: We performed microarray transcript profiling of biopsies and validated the data with RT-qPCR. We used cell line models for over expression and siRNA mediated knockdown of TOX3. Pathway analysis was applied for target gene identification and immunoprecipitation studies were used for DNA binding studies. Results: Microarray transcript profiling of 89 bladder biopsies showed a significant up-regulation of TOX3 (p<10-4) in non-muscle invasive (Ta-T1) bladder tumors compared to muscle-invasive (T2-T4) blad.. Read More»
  DOI: 10.4172/2155-9929.1000140

  Abstract PDF