Journal of Tissue Science and Engineering

Leming Shi

Publications

• Review Article
  RNA-Seq Accuracy Comprehensive Assessment, Duplicability and Knowledge Content by the Sequencing Internal Control Consortium
  Author(s): Leming ShiLeming Shi
  
  We gift primary results from the Sequencing Internal Control (SEQC) project, coordinated by the America Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites victimization reference RNA samples with intrinsical controls, we have a tendency to assess RNA sequencing (RNA-seq) performance for junction discovery and differential expression identification and compare it to microarray and quantitative PCR (qPCR) information victimization complementary metrics. in the slightest degree sequencing depths, we have a tendency to discover unannotated exon-exon junctions, with >80% valid by qPCR. We discover that measurements of relative expression area unit correct and reproducible across sites and platforms if specific filters area unit used. In distinction, RNA-seq and microarrays don't offer .. Read More»
  DOI: 10.4172/2157-7552.1000146

  Abstract PDF