GET THE APP

..

Journal of Tissue Science and Engineering

ISSN: 2157-7552

Open Access

Leming Shi


Vienna
Austria

Publications
  • Review Article
    RNA-Seq Accuracy Comprehensive Assessment, Duplicability and Knowledge Content by the Sequencing Internal Control Consortium
    Author(s): Leming ShiLeming Shi

    We gift primary results from the Sequencing Internal Control (SEQC) project, coordinated by the America Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites victimization reference RNA samples with intrinsical controls, we have a tendency to assess RNA sequencing (RNA-seq) performance for junction discovery and differential expression identification and compare it to microarray and quantitative PCR (qPCR) information victimization complementary metrics. in the slightest degree sequencing depths, we have a tendency to discover unannotated exon-exon junctions, with >80% valid by qPCR. We discover that measurements of relative expression area unit correct and reproducible across sites and platforms if specific filters area unit used. In distinction, RNA-seq and microarrays don't offer .. Read More»
    DOI: 10.4172/2157-7552.1000146

    Abstract PDF

Relevant Topics

Google Scholar citation report
Citations: 807

Journal of Tissue Science and Engineering received 807 citations as per Google Scholar report

Journal of Tissue Science and Engineering peer review process verified at publons

Indexed In

 
arrow_upward arrow_upward