Journal of Tissue Science and Engineering

Lenis AT

Publications

• Research Article
  Induced Regenerative Elastic Matrix Repair in LOXL1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse
  Author(s): Ramamurthi A, Venkataraman L, Lenis AT, Couri BM and Damaser MSRamamurthi A, Venkataraman L, Lenis AT, Couri BM and Damaser MS
  
  Impaired elastic matrix remodeling occurs in reproductive tissues, after vaginal delivery. This has been linked to development of pelvic organ prolapse (POP), for which there currently is no pharmacologic therapy. Hyaluronan oligomers and transforming growth factor beta 1 (termed elastogenic factors, EFs), have been shown to significantly enhance tropoelastin synthesis, elastic fiber assembly, and crosslinking by adult vascular smooth muscle cells(SMCs). The goal of this study was to ascertain, if these factors similarly improve the quantity and quality of elastic matrix deposition by vaginal SMCs (VSMCs) isolated from lysyl oxidase, like–1 knock out (LOXL1 KO) mouse model of POP. Cells isolated from whole vagina of a LOXL1 KO mouse (multiparous, stage 3 prolapse) were cultured and identified as SMCs, by their expression of various SMC markers. Passage 2 vaginal SMCs (VSMCs; 3&t.. Read More»
  DOI: 10.4172/2157-7552.1000120

  Abstract PDF