Alternative & Integrative Medicine

Lia Hafiyani

Publications

• Research Article
  Bufadienolides Overcome TRAIL Resistance in Breast Cancer Cells via JAK-STAT Pathway
  Author(s): Lia Hafiyani, Satoru Yokoyama, Sherif Abdelhamed, Yoshihiro Hayakawa and Ikuo SaikiLia Hafiyani, Satoru Yokoyama, Sherif Abdelhamed, Yoshihiro Hayakawa and Ikuo Saiki
  
  Aim and objective: Although the clinical significance of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been elucidated, primary or acquired resistance to TRAIL limits its efficacy in cancer patients. This study aimed to investigate bufadienolides as promising candidates to combine with TRAIL and to determine the pathway involved in overcoming TRAIL resistance in breast cancer cells. Methods: The effects of bufadienolides, cinobufagin and cinobufotalin, in combination with TRAIL were examined in TRAIL-sensitive and TRAIL-resistant breast cancer, MDA-MB-231 and MDA-MB-468 cells, respectively. Cell viability was determined using count and viability assays. Apoptotic cell death was detected by annexin-V/7-AAD staining. Silencing of MCL-1 expression was established using MCL-1 siRNA and protein expression was assessed by Western blot analysis. STAT3 localization was d.. Read More»
  DOI: 10.4172/2327-5162.1000154

  Abstract PDF