Journal of Bioprocessing & Biotechniques

Liang Zhou

Publications

• Research Article
  Extraction of Chymotrypsin from Red Perch (Sebastes marinus) Intestine Using Reverse Micelles: Optimization of the Backward Extraction Step
  Author(s): Liang Zhou, Suzanne M. Budge, Abdel E. Ghaly, Marianne S. Brooks and Deepika DaveLiang Zhou, Suzanne M. Budge, Abdel E. Ghaly, Marianne S. Brooks and Deepika Dave
  
  Fish processing waste can be used to produce valuable by-products such as chymotrypsin which has applications in the food, leather, chemical and clinical industries. In this study, a reverse micelles system of AOT/isooctane was used to extract chymotrypsin from crude aqueous extract of red perch intestine. The effects of pH and KCl concentration of the backward extraction step on the total volume (TV), volume ratio (VR), total activity (TA), enzyme activity (AE), specific activity (SA), purification fold (PF), protein concentration (Cp) and recovery yield (RY) were studied. Changing the pH from 6.5 to 8.5 and the KCl concentration from 0.5 to 2.0 M during the backward extraction step had no effects on the TV or VR. Increasing the pH from 6.5 to 7.5 increased AE, SA, Cp, PF and RY by up to 47.06%, 30.0%, 27.0%, 26.9% and 18.47%, respectively but they all then declined with further incr.. Read More»
  DOI: 10.4172/2155-9821.1000120

  Abstract PDF