Journal of Cytology & Histology

Ligia Ferreira Gomes

Publications

• Research Article
  Digital Image Processing Assessment of the Differential in vitro Antiangiogenic Effects of Dimeric and Monomeric Beta2-Glycoprotein I
  Author(s): Camila Machado, Miriela Escobedo Nicot, Carolina Nigro Stella, Sara Vaz, Cassia Prado, Durvanei Augusto Maria, Francisco Palacios Fernandez and Ligia Ferreira GomesCamila Machado, Miriela Escobedo Nicot, Carolina Nigro Stella, Sara Vaz, Cassia Prado, Durvanei Augusto Maria, Francisco Palacios Fernandez and Ligia Ferreira Gomes
  
  The β2 -glycoprotein I (β2GPI) is an endothelial cell ligand, accessible for systemic, autocrine and paracrine signaling. In vivo, β2GPI is immobilized by binding, mainly to the endothelial cell membrane heparan sulfate but also to anionic phospholipids, and functional receptors. The β2GPI was attributed antiangiogenic properties both in vitro and in vivo. This work was designed to evaluate the antiangiogenic effects of native, monomeric, and dimeric β2GPI. Monomeric as well as dimeric forms were purified from human plasma, and the native protein was obtained as a balanced mixture of both components present in human plasma. The proliferation, migration, and differentiation of Human Umbilical Vascular Endothelial Cells (HUVEC) were considered in an in vitro angiogenesis model based on tridimensional cultures and quantitative digital image processing techniques... Read More»
  

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