Biosensors & Bioelectronics

Lin Ke

Publications

• Research Article
  Immunosensor Characterization Using Impedance Spectroscopy
  Author(s): Lin Ke, Wai Kwan Lim, New Siu Yee, Xiao Di Su and Bin LiuLin Ke, Wai Kwan Lim, New Siu Yee, Xiao Di Su and Bin Liu
  
  Non-invasive and high sensitive electrochemical impedance spectroscopy (EIS) has been used for qualitative characterization of stepwise surface preparation process for constructing an immunosensor, starting from plain silicon substrate, 3-aminopropyltriethoxysilane (APTES) silanization, glutaraldehyde functionalization and analytes (rat immunoglobulin G immobilization, followed by protein G-coated gold nanoparticles), using both the horizontal direction (2 terminal) and vertical direction (3 terminal) characterization setup. The investigation demonstrated that 2-terminal setup is able to review the quality of antibody and antigen molecules bonding and distinguish the effects from the underlayers on the immuosensors; while 3-terminal setup is able to correlate the thickness and dielectric constant of each layers. It correlated well to the incubation time of preparing different sensors .. Read More»
  DOI: 10.4172/2155-6210.1000138

  Abstract PDF