Journal of Blood & Lymph

Liu Y

Publications

• Research Article
  Targeting Protein Arginine Methyltransferase 1 Blocks RBM15-MKL1 Fusion-Initiated Human Stem Cell Transformation
  Author(s): Jin S, Yao Z, Mi Y, Wang H, Yao S, Song J, Zhang P, Zhang J, Zhou W, Ma J, Guo Y and Liu YJin S, Yao Z, Mi Y, Wang H, Yao S, Song J, Zhang P, Zhang J, Zhou W, Ma J, Guo Y and Liu Y
  
  Objective: The chromosome translocation t (1;22), which generates the RBM15-MKL1 fusion gene, is found in approximately 10% of pediatric acute megakaryoblastic leukemia cases. Given that PRMT1 downregulation is critical for megakaryocyte differentiation, we propose the use of the PRMT1 inhibitor furamidine to stimulate RBM15- MKL1-transformed human cord blood cells to undergo megakaryocyte differentiation. Materials and methods: Human CD34+ cells were purified from umbilical cord blood with anti-CD34 magnetic beads. Lentivirus-infected CD34+ cells were sorted using flow cytometry. The methylcellulose colony replating assay was performed to evaluate the transformation efficiency. Cell viability was calculated using a CellTiter-Glo® luminescent cell viability assay kit. Results: The simultaneous transduction of RBM15-MKL1 and MPLW515L, a mutated MPL gene found in AMKL patients, int.. Read More»
  DOI: 10.4172/2165-7831.1000158

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