Molecular Biomarkers & Diagnosis

Lloyd Hutchinson

Publications

• Research Article
  The Use of Immunoglobulin Gene Rearrangement Polymerase Chain Reaction Assays for Detection of B-Cell Clonality for Plasma Cell Neoplasms Using Novel PCR Primers
  Author(s): Lloyd Hutchinson, Paul J Lee, Bruce A Woda, Ediz F Cosar, Mandi-Lee Caporelli BS and Xiuling MengLloyd Hutchinson, Paul J Lee, Bruce A Woda, Ediz F Cosar, Mandi-Lee Caporelli BS and Xiuling Meng
  
  We have designed a standardized protocol with multiplex-primer sets capable of detecting majority IG kappa (IGK) and IG lambda (IGL) light chain rearrangements in plasma cell neoplasms (PCN). Thirty primers were combined in three multiplexed PCR reactions to target IGK, KDE and IGL rearrangements. Variable region (V) primers were designed to prevent “primer dimers”, provide matching melting temperatures (Tm), minimize amplicon size, and optimize sequencing time. Amplicons were subjected to capillary gel electrophoresis for analysis. In a discovery series, we tested 37 plasma cells neoplasms PCN (28 PCNs at diagnosis and 9 PCNs post-treatment). The assay investigated an additional 52 prospective PCN cases in the validation series. Results were compared to bone marrow morphology, immunohistochemical (IHC), flow cytometry data, and standard IGH FRIII gene rearrangement assay... Read More»
  DOI: 10.4172/2155-9929.1000302

  Abstract PDF