Metabolomics:Open Access

Lu C

Publications

• Research Article
  Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
  Author(s): Gao D, Liu N, Li Y, Zhang Y, Liu G, Xiao G and Lu CGao D, Liu N, Li Y, Zhang Y, Liu G, Xiao G and Lu C
  
  Eha is a virulence regulator associated with the replication of Edwardsiella tarda within RAW264.7 macrophages. Eha is required for the bacteria to resist the acid and oxidative stress in macrophages. We herein demonstrate that Eha regulates the resistance of this bacterium against acidification in macrophages and explain the underlying molecular mechanism. Firstly, to find an acid or oxidative condition to induce the strongest Eha activities, we constructed pMP220- Pehalac Z plasmid and inspected the lac Z expression regulated by Eha by using a β-galactosidase assay. At exposure of pH6.3 medium 2 h, whole transcriptomic profiling of the wild type and mutant were performed by RNA-sequencing. We identified.. Read More»
  DOI: 10.4172/2153-0769.1000196

  Abstract PDF