Joint E, National Autonomous University of Mexico (UNAM), DF 04510
Mexico
Research Article
Properties of the Entomoparasitic Nematodes (Heterorhabditis bacteriophora) Liquid Culture using a Helicoidal Ribbon Agitator as Rheometric System
Author(s): Diola-Marina Nuñez-Ramirez, Luis Medina-Torres, Fausto Calderas and Guadalupe Sanchez-OlivaresDiola-Marina Nuñez-Ramirez, Luis Medina-Torres, Fausto Calderas and Guadalupe Sanchez-Olivares
The rheological parameters: flow behaviour index n, flow consistency index K and effective viscosity ηe were estimated for the entomoparasitic nematodes Heterorhabditis bacteriophora liquid broth at different culture maturation times. The nematode or nematodes were cultivated during 20 days in a bioreactor, and the growth media inside the bioreactor was enriched with protein and fat sources. Rheological parameters for the heterogeneous suspension were estimated with mixer principles employing a helical ribbon agitator fixed to a rheometer. As the culture matured, n decreased from 0.8 to 0.2 (-) and K increased up to 1200 mPa•sn; ηe showed a non-Newtonian (n<1) behaviour, ηe reached peak values of 0.32 Pa•s for a rotational speed of 0.5 revolutions per second (rps) and 0.048 Pa•s for 2.5 rps. Rheological properties reported here could be more reliabl.. Read More»
DOI:
10.4172/2155-9821.1000207
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report