Journal of Bioanalysis & Biomedicine

M Rohan Fernando

Publications

• Research Article
  Viral Load Stability of an RNA virus In Stabilized Blood Samples
  Author(s): Jianbing Qin, Kausik Das, Eunhee Kwon, Veenu Minhas, Susan Swindells, Charles Wood and M Rohan FernandoJianbing Qin, Kausik Das, Eunhee Kwon, Veenu Minhas, Susan Swindells, Charles Wood and M Rohan Fernando
  
  Background: The stability of viral load in a patient blood sample during storage and shipping is an important pre-analytical variable that affects the accuracy of viral pathogen quantitation. Therefore, there is a need for developing a blood collection tube with stabilizing reagents which can not only inactivate the viral agent but also stabilize the viral nucleic acids for accurate viral load determination in blood samples post blood draw. This study was undertaken to investigate the ability of Cyto-Chex® BCT (BCT) blood collection device to stabilize the nucleic acids of the Mason-Pfizer monkey virus, an RNA virus, in collected blood specimens during storage and shipping for viral load determination at a later time point. Findings: Blood was drawn from each donor into K3EDTA and BCT tubes, spiked with Mason-Pfizer monkey virus particle.. Read More»
  DOI: 10.4172/1948-593X.1000112

  Abstract PDF