Molecular Biomarkers & Diagnosis

Madhukar Rai

Publications

• Research Article
  Cloning, Expression and Purification of L. Donovani Specific Antigen for Serodiagnosis of Visceral Leishmaniasis
  Author(s): Dinesh Kumar, Puja Tiwary, Anuradha Dube, Jaya Chakravarty, Madhukar Rai and Shyam SundarDinesh Kumar, Puja Tiwary, Anuradha Dube, Jaya Chakravarty, Madhukar Rai and Shyam Sundar
  
  Background: Rapid diagnostic test using rk39 antigen is widely used for visceral leishmaniasis. However it detects anti-rk39 antibodies in 20-32% of endemic healthy individuals. In search for a better biomarker of infection, we identified a protein of molecular weight 70 kDa (BHUP1), specifically recognized by sera of visceral leishmaniasis (VL) patients. Methods: The protein was cloned as His-tagged fusion protein and purified. We evaluated the sensitivity and specificity of this protein in an enzyme linked immunosorbant assay (ELISA) format in comparison to the rk39 antigen using sera collected from various groups of individuals. Results: The sensitivity of rBHUP1 was 96.5% compared to 98.8% with rk39. For healthy controls from non endemic and endemic regions, the specificity of rBHUP1 was 100% and 95.6% compared to 100% and 84.9% for rk39, respectively. For other i.. Read More»
  DOI: 10.4172/2155-9929.1000141

  Abstract PDF