United Kingdom
Research Article
Evaluation of Low Trace DNA Recovery Techniques from Ridged Surfaces
Author(s): Graham Williams, Manohar Pandre, Waseeh Ahmed, Emma Beasley, Emma Omelia, Damian World and Holly YuGraham Williams, Manohar Pandre, Waseeh Ahmed, Emma Beasley, Emma Omelia, Damian World and Holly Yu
The first step in the DNA profiling process is the recovery of DNA from the crime scene or item. Such surfaces can be varying with absorbent and non-absorbent surfaces. Ridged surfaces, such as those encountered on bottletops or firearms, are also common. Although low trace DNA or “touch DNA” is problematic in forensic casework, ridged surfaces should be a more effective surface for the recovery of such DNA due their abrasive nature. The aim of this study was to evaluate six recovery techniques; single wet swab, dry-wet double swab, wet-dry double swab, flocked swab, PCR square, and tape lifts. 36 falcon tubes with screw top lids were sterilised before DNA was deposited on the screw-top lids. Each of the recovery techniques were then utilised on 6 screw-top lids. All recovery media then underwent standard DNA extraction pr.. Read More»
DOI:
10.4172/2157-7145.1000199
Journal of Forensic Research received 1817 citations as per Google Scholar report