Journal of Cytology & Histology

Manuel Garrosa García

Publications

• Research Article
  Addition of Insulin-like Growth Factors (IGF-1) for the Regeneration of Critical Defects in Peripheral Nerve Injuries. Biological Findings
  Author(s): Belén García Medrano, Clarisa Simón Pérez, Patricia Barrio Sanz, Manuel Garrosa García, Manuel José Gayoso Rodríguez and Miguel Ángel Martín FerreroBelén García Medrano, Clarisa Simón Pérez, Patricia Barrio Sanz, Manuel Garrosa García, Manuel José Gayoso Rodríguez and Miguel Ángel Martín Ferrero
  
  Introduction: The purpose is analyze the biological and cellular regeneration of non-repairable lesions of peripheral nerve by muscle graft enhanced with growth factors. Methods: Controlled clinical experimental trial, level I evidence. Critical peripheral nerve injuries repair through acellular muscle graft alone versus its enrichment with IGF-1 (10 mg/ml mecasermin). Clinical and functional control for 3 months. Animals were sacrificed at 90-100 days, obtaining samples for macro and microscopy with blue toluidine staining, hematoxylin-eosin and Masson trichrome. Results: Regenerating axons can pass through a defect length of 15 mm with the addition of an acellular skeletal muscle graft and repopulate the distal nerve. Basal lamina tubes, acting as matrices for the axonal growth, appears that sensory recovery exceeds t.. Read More»
  DOI: 10.4172/2157-7099.1000221

  Abstract PDF