Biosensors & Bioelectronics

Marcus D Hughes

Publications

• Research Article
  EDC-Mediated Oligonucleotide Immobilization on a Long Period Grating Optical Biosensor
  Author(s): Xianfeng Chen, Chen Liu, Marcus D Hughes, David A Nagel, Anna V Hine and Lin ZhangXianfeng Chen, Chen Liu, Marcus D Hughes, David A Nagel, Anna V Hine and Lin Zhang
  
  We present the development and simplification of label-free fiber optic biosensors based on immobilization of oligonucleotides on dual-peak long period gratings (dLPGs). This improvement is the result of a simplification of biofunctionalization methodology. A one-step 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated reaction has been developed for the straightforward immobilization of unmodified oligonucleotides on the glass fiber surface along the grating region, leading to covalent attachment of a 5´-phosphorylated probe oligonucleotide to the amino-derivatized fiber grating surface. Immobilization is achieved via a 5´phosphate-specific linkage, leaving the remainder of the oligonucleotide accessible for binding reactions. The dLPG has been tested in different external media to demonstrate its inherent ultrahigh sensitivity to the .. Read More»
  DOI: 10.4172/2155-6210.1000173

  Abstract PDF