Environmental & Analytical Toxicology

Maria Aparecida Marin-Morales

Publications

• Research Article
  The use of the Cytogenetic to Identify Mechanisms of Action of an Azo Dye in Allium Cepa Meristematic Cells
  Author(s): Bruna de Campos Ventura-Camargo, Patrícia Pasquali Parise Maltempi and Maria Aparecida Marin-MoralesBruna de Campos Ventura-Camargo, Patrícia Pasquali Parise Maltempi and Maria Aparecida Marin-Morales
  
  In the present study, cytotoxic, genotoxic and mutagenic actions of different concentrations (1, 10, 100 and 1000 μg/L) of an azo dye (BDCP – Black Dye Commercial Product) were evaluated using different cytogenetic techniques [(conventional dye, C banding, NOR banding, base-specific fluorochrome banding and fluorescent in situ hybridization (FISH)] applied to the Allium cepa test-organism. The use of conventional cytogenetic staining allowed us to determine that the azo dye induced cell death, chromosomal aberrations, nuclear alteration and micronuclei. By means of chromosome bandings and the FISH technique, it was possible to notice both cell and nucleolar alterations induced by BDCP, whose effects, in a general way, were not specific for any chromosome sites. The abnormalities observed made it possible to infer both aneugenic and clastogenic actions caused by the dye anal.. Read More»
  DOI: 10.4172/2161-0525.1000109

  Abstract PDF