Nuclear Medicine & Radiation Therapy

Marie Boyd

Publications

• Research Article
  Inhibition of the BER Factor APE1 Disrupts Repair of Double-Strand DNA Damage in Cells Treated with Low Dose-Rate, but Not High Dose-Rate XRadiation
  Author(s): Anthony Gerald McCluskey and Marie BoydAnthony Gerald McCluskey and Marie Boyd
  
  Introduction: Radiotherapy is utilised in the treatment of many cancers, but its efficacy is limited by normal tissue toxicity and new radiotherapy techniques are thus urgently sought. The AP endonuclease APE1 is involved in repair of single strand DNA damage through the break excision repair (BER) pathway and altered levels of APE1 have been found in some cancers. In this study, we investigated the effects of APE1 inhibition, using the APE1-specific inhibitor CRT0044876 (CRT), in tumour cells following exposure to either high dose-rate (HDR) or low dose-rate (LDR) X-irradiation. Materials and Methods: Treatment efficacy was assessed by clonogenic assay followed by isobologram analysis to assess potential synergy. Cell cycle distribution was assessed by propidium iodide staining followed by flow cytometry. Induction of DNA damage and repair w.. Read More»
  DOI: 10.4172/2155-9619.1000269

  Abstract PDF