Journal of Bioprocessing & Biotechniques

Mark G Lefsrud

Publications

• Research Article
  Fermentation Monitoring of a Co-Culture Process with Saccharomyces cerevisiae and Scheffersomyces stipitis Using Shotgun Proteomics
  Author(s): Eric L Huang and Mark G LefsrudEric L Huang and Mark G Lefsrud
  
  Co-culture processes present the opportunity to produce value-added products from economical raw materials, but there lacks a high-throughput fermentation monitoring technique to study the temporal physiology of fermentation organisms in co-culture processes. In this study, we applied shotgun proteomics to investigate a co-culture process of Saccharomyces cerevisiae and Scheffersomyces stipitis, and we monitored the fermentation until glucose depletion. Three time points were taken for proteomics analysis at 11.5 hour, 18.5 hour and 32 hour, representing transition into diauxic shift. Using label-free quantitation, we observed cellular dynamic within 20-hour time frame. We distinguished the proteome between two yeasts, and the most abundant proteins of S. stipitis and S. cerevisiae contained expected processes of glycolytic enzymes, histo.. Read More»
  DOI: 10.4172/2155-9821.1000144

  Abstract PDF