Journal of Tissue Science and Engineering

Masahiro Okumura

Publications

• Research Article
  Pentosan Polysulfate Sodium Restores the Phenotype of Dedifferentiated Monolayer Canine Articular Chondrocytes Cultured in Alginate Beads
  Author(s): Eugene Bwalya, Sangho Kim, Jing Fang, HM Suranji Wijekoon, Kenji Hosoya and Masahiro OkumuraEugene Bwalya, Sangho Kim, Jing Fang, HM Suranji Wijekoon, Kenji Hosoya and Masahiro Okumura
  
  Autologous chondrocyte transplantation is a promising option for the repair of isolated osteoarthritic cartilage lesions that requires isolation and expansion of chondrocytes from a small cartilage biopsy prior to implantation. However, when cultured in vitro, chondrocytes lose their stable phenotype and dedifferentiate to fibroblastic-like cells. The study investigated the potential of pentosan polysulfate (PPS) sodium to restore the phenotype of dedifferentiated monolayer articular chondrocytes. Canine articular chondrocytes isolated from four cartilage samples were culture expanded to establish primary culture. First passage chondrocytes were cultured as alginate beads for 18 days under normoxia in PPS concentrations of 0, 1, 5, 15 and 40 μg/mL in 20% DMEM. Effect of PPS on type I, II and X collagen, aggrecan and Runx2 gene expression were evaluated by real-time PCR. Runx2, HI.. Read More»
  DOI: 10.4172/2157-7552.1000218

  Abstract PDF