Journal of Forensic Research

Mavis Swerdel

Publications

• Research Article
  STR Profiling of Human Cell Lines: Challenges and Possible Solutions to the Growing Problem
  Author(s): Rixun Fang, Jaiprakash G. Shewale, Vivian T. Nguyen, Holly Cardoso, Mavis Swerdel, Ronald P.Hart and Manohar R. FurtadoRixun Fang, Jaiprakash G. Shewale, Vivian T. Nguyen, Holly Cardoso, Mavis Swerdel, Ronald P.Hart and Manohar R. Furtado
  
  Genomic DNA preparations from 60 human cell lines from the National Cancer Institute (NCI), 48 human cell lines from American Type Culture Collection (ATCC) and 19 embryonic cell lines were profiled for autosomal short tandem repeat (STR) loci using the AmpFÃ?¢Ã?â??Ã?â??STR ® Identifiler ® kit. Each DNA sample was profiled at least twice to ensure consistency and reproducibility of results. The resulting STR profiles were compared with the STR profiles in the database of ATCC. The allele calls for the common loci between the Identifiler ® kit and the database were identical except for one DNA sample, which we attribute to amplification artifacts. We have observed a high percentage of the STR loci exhibiting allelic imbalance. Certain STR loci for some cell lines exhibited 3 or more alleles. This type of observation can result from a unique profile for a given ce.. Read More»
  DOI: 10.4172/2157-7145.S2-005

  Abstract PDF