China
Research Article
Molecular Characterization of CTX-M Producing Salmonella Isolates with Concurrent Resistance to Ciprofloxacin and Cefotaxime from Slaughtered Chicken Carcasses in Qingdao, China
Author(s): Wei Wang, Jin Xu, Li Zhao, Yujie Hu, Yao Bai, Xin Gan, Yinping Dong, Zixin Peng, Shaofei Yan, Menghan Li, Fengqin Li and Séamus FanningWei Wang, Jin Xu, Li Zhao, Yujie Hu, Yao Bai, Xin Gan, Yinping Dong, Zixin Peng, Shaofei Yan, Menghan Li, Fengqin Li and Séamus Fanning
Objectives: To describe the molecular characterization of CTX-M ESBL (Extended-spectrum Beta-lactamase)- producing Salmonella co-resistant ciprofloxacin and cefotaxime from chicken carcasses in 2014 in Qingdao, China.
Methods: A total of 62 Salmonella isolates were confirmed as ESBLs by a combination disc diffusion test from 355 Salmonella isolates studied. Antimicrobial susceptibility was determined by the broth dilution method. CTX-M subtypes, Quinolone Resistance-determining Regions (QRDRs), Plasmid-mediated Quinolone Resistance (PMQR) determinants and Multi-locus Sequence Typing (MLST) were identified using PCR (Polymerase Chain Reaction) and sequencing. Plasmids were characterized by S1-nuclease PFGE, PCR-based replicon typing and transformation. Broth mating assays were carried out for all isolates to determine .. Read More»
DOI:
10.4172/2472-1212.1000185
Journal of Antimicrobial Agents received 444 citations as per Google Scholar report