Egypt
Research Article
Age-Dependent Gene Expression of Blow Fly Lucilia cuprina (Diptera: Calliphoridae) during Egg Development Improving Age Estimation in Forensic Entomology
Author(s): Abdel-Ghaffer H, Mennatallah Abd-Elmottelb and Amira ZakyAbdel-Ghaffer H, Mennatallah Abd-Elmottelb and Amira Zaky
Estimating the age of immature stages of necrophagous flies developing on corps, may provide clues for understanding the minimum post-mortem intervals (PMI) to aid death investigation. It can be hard to comprehend precisely the interval age of developmental stage that does not increase in size including egg. In this study we investigated the feasibility of predicting egg age from ovi-position to hatching over 2 hour’s intervals of egg development. The expression profile of three genes, cuticle I, Ecdysone receptor (EcR) and chymotrypsin of lucilia cuprina (Diptera: Calliphoridae) were determined at 2, 6, and 8 hours of egg development. Semi-quantitative reverse transcriptase (RT)-PCR were applied for profiling of indicated genes expressions from eggs of different developmental stages by designing specific primers sets. Target tr.. Read More»
DOI:
10.4172/2157-7145.1000238
Journal of Forensic Research received 2328 citations as per Google Scholar report