Molecular Biomarkers & Diagnosis

Michael A. Kennedy

Publications

• Research Article
  Metabolic Profiling Comparison of Human Pancreatic Ductal Epithelial Cells and Three Pancreatic Cancer Cell Lines using NMR Based Metabonomics
  Author(s): Miki Watanabe, Sulaiman Sheriff, Kenneth B. Lewis, Junho Cho, Stuart L. Tinch, Ambikaipakan Balasubramaniam and Michael A. KennedyMiki Watanabe, Sulaiman Sheriff, Kenneth B. Lewis, Junho Cho, Stuart L. Tinch, Ambikaipakan Balasubramaniam and Michael A. Kennedy
  
  Metabolic profiles of hydrophilic and lipophilic cell extracts from three cancer cell lines, Miapaca-2, Panc-1 and AsPC-1, and a non-cancerous pancreatic ductal epithelial cell line, H6C7, were examined by proton nuclear magnetic resonance spectroscopy. Over twenty five hydrophilic metabolites were identified by principal component and statistical significance analyses as distinguishing the four cell types. Fifteen metabolites were identified with significantly altered concentrations in all cancer cells, e.g. absence of phosphatidylgrycerol and phosphatidylcholine, and increased phosphatidylethanolamine and cholesterols. Altered concentrations of metabolites involved in glycerophospholipid metabolism, lipopolysaccharide and fatty acid biosynthesis indicated differences in cellular membrane composition between non-cancerous and cancer cells. In addition to cancer specific metabolites, .. Read More»
  DOI: 10.4172/2155-9929.S3-002

  Abstract PDF