Journal of Material Sciences & Engineering

Michael Cho

Publications

• Research Article
  Characterization of Correlated Calcium Dynamics in Astrocytes in PCL Scaffold: Application of Wavelet Transform Coherence
  Author(s): Bo Chen, Fenghua Tian, Nicole Hashemi, Marilyn McNamara and Michael ChoBo Chen, Fenghua Tian, Nicole Hashemi, Marilyn McNamara and Michael Cho
  
  While 2D culture models have been used extensively to elucidate the cell-to-cell communication, they do not recapitulate fully the 3D characteristics of microenvironment in vivo, e.g., polarized cell attachment and generally confer a considerably stiffer substrate than the endogenous extracellular matrix. Development of fibrous scaffolds that can better mimic the native microenvironment and improve the spatial arrangement of seeded cells should foster experimental strategies to monitor and determine the 3D cell-to-cell communication. In this study, poly(ε- caprolactone) (PCL) fibers were fabricated in different sizes using a microfluidic platform and spatially arranged to create a suitable 3D microenvironment in order to investigate the cell viability and calcium signaling in mouse astrocytes. A powerful algorithm, referred to as wavelet transform coherence (WTC), was applied to .. Read More»
  DOI: 10.4172/2169-0022.1000453

  Abstract PDF