Molecular Biomarkers & Diagnosis

Michael J Domanico

Publications

• Research Article
  Aberrantly Methylated Gene Marker Levels in Stool: Effects of Demographic, Exposure, Body Mass, and Other Patient Characteristics
  Author(s): David A Ahlquist, William R Taylor, Tracy C Yab, Mary E Devens, Douglas W Mahoney, Lisa A Boardman, Steven N Thibodeau, Hongzhi Zou, Michael J Domanico, Barry M Berger and Graham P LidgardDavid A Ahlquist, William R Taylor, Tracy C Yab, Mary E Devens, Douglas W Mahoney, Lisa A Boardman, Steven N Thibodeau, Hongzhi Zou, Michael J Domanico, Barry M Berger and Graham P Lidgard
  
  Background: Selected aberrantly methylated genes represent sensitive candidate stool markers for colorectal cancer (CRC) screening. We assessed the impact of demographic, exposure, body mass, and other patient variables on stool levels of highly informative methylated gene markers — BMP3, NDRG4, vimentin, and TFPI2. Methods: We studied freezer-archived stools from 500 patients with normal colonoscopy (median age 64 (range 44-85); 53% women). On supernatants from thawed aliquots, target gene sequences were purified by hybrid capture; bisulfite treated, and assayed using the analytically-sensitive QuARTS method (quantitative allele-specific real-time target and signal amplification). The reference human gene β-actin was assayed along with the 4 methylated genes. Results: Only age significantly influenced all methylated marker levels in stool (p<0.0001 for each). The relati.. Read More»
  DOI: 10.4172/2155-9929.1000133

  Abstract PDF