Biosensors & Bioelectronics

Michael R Caplan

Publications

• Research Article
  Electrochemical-Nucleic Acid Detection with Enhanced Specificity and Sensitivity
  Author(s): Aldin Malkoc, Edgar Sanchez, Michael R Caplan and Jeffrey T La BelleAldin Malkoc, Edgar Sanchez, Michael R Caplan and Jeffrey T La Belle
  
  Development of a rapid and label-free Electrochemical Impedance Spectroscopy (EIS) biosensor for nucleic acid detection based on sensitivity and specifies was proposed to fill the technology gap between rapid and portable detection of DNA sequences. Current biosensors have inherent limitations to sensitivity and specificity due to the monovalent character of the detection method. These limitations were significantly improved upon by constructing cooperative probes, called Tentacle Probes™ (TP), which utilize divalent binding to achieve both high sensitivity as well as extremely high specificity. Immobilization of Tentacle Probe onto gold disk electrode produced statistical significance, with p<3.0*10-13. Tentacle Probe with EIS detection was able to detect a difference between the B. AnthracisgyrA gene and B. Cereus gyrA Single Nucleotide Polymorphism sequence at a lower limi.. Read More»
  DOI: 10.4172/2155-6210.1000171

  Abstract PDF