Journal of Cytology & Histology

Michela Zago

Publications

• Research Article
  De-Regulation of Extracellular Matrix Proteins in Human Fibroblasts after Long-term and Low Concentrations of HEMA Exposition
  Author(s): Gabriella Teti, Michela Zago, Sandra Durante, Stefano Focaroli, Antonio Mazzotti, Viviana Salvatore, Matteo Cadossi and Mirella FalconiGabriella Teti, Michela Zago, Sandra Durante, Stefano Focaroli, Antonio Mazzotti, Viviana Salvatore, Matteo Cadossi and Mirella Falconi
  
  Background: 2 - hydroxyethyl methacrylate (HEMA) is one of the common components of most resin-based dental materials. Various studies have shown that HEMA can diffuse through dentin due to its low molecular weight and its hydrophilicity, and can affect the underlying odontoblast, cell division and activity. In this work, we have studied the influence of HEMA in regulating the expression of pro-collagen α1 type I and tenascin - C proteins in human fibroblasts after long term and low concentrations of HEMA. Methods: Human dental pulp cells were exposed to 0.1 mM and 0.5 mM for 1, 3, 5, 7, and 15 days. MTT assay, immunofluorescence and western blot analysis were carried out to investigate cell viability and modification in collagen type I and tenascin – C protein expression. Results: MTT assay showed an high .. Read More»
  DOI: 10.4172/2157-7099.1000235

  Abstract PDF