Journal of Bioanalysis & Biomedicine

Miguel Ángel Galván Morales

Publications

• Research Article
  Effect of Human Beta Defensin-2 in Epithelial Cell Lines Infected with Respiratory Viruses
  Author(s): Miguel Ángel Galván Morales, Alejandro Escobar Gutiérrez, Dora Patricia Rosete Olvera and Carlos Cabello GutiérrezMiguel Ángel Galván Morales, Alejandro Escobar Gutiérrez, Dora Patricia Rosete Olvera and Carlos Cabello Gutiérrez
  
  β-defensins are a family of antimicrobial molecules involved in inflammatory processes and infections. In human airways, β-defensin-2 (hβD-2) is the best characterized in bacterial and fungal infections; however, it has been insufficiently studied in viral infections. The respiratory syncytial virus (RSV) and adenoviruses (ADV) are important agents of acute respiratory infections. The aim of this study was to measure in vitro the production and antiviral activity of hβD-2 in HEp-2 cells and A549 cells infected with ADV and RSV; hβD-2 production at different times was assessed by RT-PCR, and its presence by immunodetection assay (Western blot) using antibodies anti-hβD-2. The effect of this defensin on viral replication was determined using recombinant hβD-2 in plaque assays. The results revealed that in the cell lines pro.. Read More»
  DOI: 10.4172/1948-593X.1000135

  Abstract PDF