Chemical Sciences Journal

Miotti N

Publications

• Research Article
  Ethidium Bromide and SYBR Green I Interact with Lipid Micelles
  Author(s): Garro AG, Velez PS, Miotti N, Alasino RV and Beltramo DMGarro AG, Velez PS, Miotti N, Alasino RV and Beltramo DM
  
  Ethidium Bromide and SYBR Green I are usual examples of ligands that specifically binds to DNA by intercalation. Here we describe that micelles of gangliosides, a lipidic family of sialoglycosphingolipids which are the most important lipids in central nervous system, are able to interact with both, EtBr and SYBR Green I and fluoresce after UV light excitation. The interaction is not affected by the presence of high saline concentration (1M NaCl), a condition which dissociate potential electrostatic interaction between amino and carboxylic acid groups present in EtBr and GM1 respectively; instead, increase the fluorescense of EtBr/GM1 micelles. This result suggests that the EtBr is located inside the hydrophobic core of the micelle. Moreover, the SYBR Green I associated ganglioside micelles does not emit green light as with DNA, but there was a change in the light spectrum towards high.. Read More»
  

  Abstract PDF