Molecular Biology: Open Access

Mohammadamin Almasi

Publications

• Research Article
  Establishment and Application of a Reverse Transcription Loop-mediated Isothermal Amplification Assay for Detection of Grapevine Fanleaf Virus
  Author(s): Mohammadamin AlmasiMohammadamin Almasi
  
  Grapevine degeneration in grapevines caused by Grapevine Fanleaf Virus (GFLV) has been documented in many viticulture regions worldwide. It is one of the major economically important virus diseases affecting the longevity of grapevines and reducing the fruit yield and fruit quality. To diminish the time required for some diagnostic assays, including reverse transcription polymerase chain reaction (RT-PCR), reverse transcription loop-mediated isothermal amplification (RT-LAMP) and also double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) into a minimum level, an innovative immunocapture RT-LAMP (IC-RT-LAMP) and immunocapture RT-PCR (IC-RT-PCR) protocol on the basis of GFLV genome were used and the reaction conditions were optimized for rapid detection of GFLV. In this regard, DAS-ELISA was employed first to validate the existence of the virus. All six RT-LAMP primers .. Read More»
  DOI: 10.4172/2168-9547.1000149

  Abstract PDF