Molecular Biology: Open Access

Narayanan Ramasubbu

Publications

• Research Article
  Pub: Probing the Interaction of Human Salivary Alpha-Amylase and Amylase Binding Protein A (AbpA) of Streptococcus gordonii
  Author(s): Prerna Gopal, Chandran Ragunath, Vishal Vyas, Mayilvahanan Shanmugam and Narayanan RamasubbuPrerna Gopal, Chandran Ragunath, Vishal Vyas, Mayilvahanan Shanmugam and Narayanan Ramasubbu
  
  Amylase binding protein A (AbpA) of Streptococcus gordonii serves as a major receptor for human salivary α-amylase (HSAmy), the predominant enzyme in human salivary secretions, to bind to the bacterial cell surface. On enamel surfaces, the binding of AbpA to HSAmy renders S. gordonii act as a scaffold for other oral bacteria to attach to the acquired enamel pellicle leading to complex bacterial communities and invasion of host tissues. While the role of AbpA in adhesion, starch metabolism and biofilm formation in influencing the ecology of the oral biofilms has been established, the structure function relationships of AbpA are yet to be defined. Since distally located aromatic residues of HSAmy are involved in the interaction with AbpA, we hypothesized that AbpA might use separate structural regions to bind to HSAmy. To test this, several deletion mutants of Abp.. Read More»
  DOI: 10.4172/2168-9547.1000111

  Abstract PDF