Tanzania
Research Article
Flow Cytometry Monitors Xylitol Metabolism of Streptococcus mitis
Author(s): Prabani Dissanayake, Nardhy Gomez- Lopez, Gail Czarnecki and Sunil PalchaudhuriPrabani Dissanayake, Nardhy Gomez- Lopez, Gail Czarnecki and Sunil Palchaudhuri
In this work we are presenting our novel adaptation of flow cytometry for the determination of change of diplococcic Gram-positive Streptococcus mitis population heterogeneity by their xylitol metabolism. The inherent population heterogeneity of the bacteria due to their growth in-chains of varying lengths was grouped into three different groups by flow cytometric analysis, designated as gates P1, P2 and P3. Gate P1 consists of the bacterial subpopulation with minimum cell wall thickness and therefore the least side scattering, while gate P3 consists of the subpopulation with the most side scattering (corresponding to the thick bacterial cell wall). According to our results gate P1 contains bacteria in long-chains, while P3 contains individual bacteria. When these diplococcic bacteria were grown in the presence of 2% xylitol more homogeneous population was seen as P1 gate was populate.. Read More»
Molecular and Genetic Medicine received 3919 citations as per Google Scholar report
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