Journal of Bioanalysis & Biomedicine

Oliveira CostaI

Publications

• Research Article
  Cimetidine Quantification in Human Plasma by High-performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry. Application to a Comparative Pharmacokinetics Study
  Author(s): Moreno RA, Oliveira CostaI, Brum Junior L, Sverdloff CE, Domingues CC, Borges DC, Oliveira RA and Borges NCMoreno RA, Oliveira CostaI, Brum Junior L, Sverdloff CE, Domingues CC, Borges DC, Oliveira RA and Borges NC
  
  A specific, fast and sensitive LC–MS/MS assay was d e- veloped for the determination of cimetidine in huma n plasma using nizatidine as the internal standard (I S). The limit of quantification was 5.0 ng/ml and the metho d was linear in the range of 5.0 to 5000 ng/ml. The cimet idine and IS retention times were 1.35±0.3 and 1.40±0.03 min, respectively. Method intra-batch precision and accu racy ranged from 2.0 to 5.4%, and 92.1 to 103.7%, respec tively. Inter-batch precision ranged from 4.2 to 6.3%, whil e In- ter-batch accuracy ranged from 97.0 to 106.6%. The analytical method was applied to evaluate the p har- macokinetic and relative bioavailability of two dif ferent pharmaceutical formulations containing 400 mg of cimetidine containing. This study evaluated 29 volu nteers in a randomized, 2-period crossover .. Read More»
  DOI: 10.4172/1948-593X.1000002

  Abstract PDF