Shibin El-Kom
Egypt
Research Article
Phenotypic and Genotypic Diversity of Rhizobia Nodulating Faba Bean from Various Egyptian Locations
Author(s): Omar A Hewedy, Ragaa A Eissa, Elzanaty AM, Nagaty HH and Abd Elbary MIOmar A Hewedy, Ragaa A Eissa, Elzanaty AM, Nagaty HH and Abd Elbary MI
Thirteen Rhizobial isolates from root nodules of Faba beans (Vicia faba L.) were obtained from eleven governorates, representing different agro-ecological, agro-climatic and soil conditions in Egypt. A polyphasic approach, including phenotypic and genotypic techniques were used to study their diversity. Rhizobium isolates were examined for their ability to tolerate salt stress and antibiotic resistance. We used four NaCl concentrations ranging from 0.1%, 1%, 3% and 5% in liquid media and plates, moreover we used 24 different antibiotic disks to determine resistance or sensitive among the tested isolates on plates. The obtained results after salt tolerance and antibiotic response of the R. leguminosarium strains showed that the isolate No. RL9 was the superior strain for salt tolerance . The following strains for salt tolerance were the isolates (RL4) and (RL12), although the isolate N.. Read More»
DOI:
10.4172/2155-9821.1000170
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report