Kenya
Research Article
Efficient Plant Regeneration of Selected Kenyan Sweetpotato (Ipomoea batatas (L.) Lam.) Cultivars through Somatic Embryogenesis
Author(s): Wilton Mbinda, Sylvester Anami, Omwoyo Ombori, Christina Dixelius and Richard OduorWilton Mbinda, Sylvester Anami, Omwoyo Ombori, Christina Dixelius and Richard Oduor
Sweetpotato is an important food crop in the world as well as in Kenya. Various fungal, and viral diseases are major constraints in its production and are currently threatening the sweetpotato production in sub-Saharan Africa. Genetic engineering offers significant potential for the crop’s genetic improvement. However, this is limited by the low efï¬Â?ciency and strong genotype dependency in tissue culture. This study aimed to establish an efficient somatic embryogenesis and plant regeneration system using shoot apical meristem explants of sweetpotato. Three sweetpotato cultivars that are widely grown in Kenya; KSP36, Kemb36 and Mweu mutheke along with an exotic model cultivar Jewel were evaluated. The maximum somatic embryogenic induction, at 96.72%, was obtained from explants cultured on Linsmaier and Skoog salts and vitamins medium supplemented with 0.5 mg/l.. Read More»
DOI:
10.4172/2157-7552.1000176
Journal of Tissue Science and Engineering received 807 citations as per Google Scholar report