Journal of Cytology & Histology

Otakar Sohnel

Publications

• Research Article
  Ultrafine Structure of Human Aortic Valve Calcific Deposits
  Author(s): Felix Grases, Otakar Sohnel and Marketa ZelenkovaFelix Grases, Otakar Sohnel and Marketa Zelenkova
  
  Objective: To elucidate the mechanism of formation of calcific deposits inside the human natural heart valve based on their ultra-fine structure on nanometer scale observed by atomic force microscope. Methods: Cross-sections of an aortic valve calcification were observed by scanning electron microscope. Thin slices several micrometeres thick from the same deposit were observed by atomic force microscopy in the Peak Force Imagining mode providing topographic, adhesion and quantitative nanoscale elastic modulus maps of the surface. Results: Most of the aortic valve calcification was composed of large blocks of mostly compact matter, porous on the microscopic scale. The mineral blocks consisted mainly of closely arranged elongated needle- and plate-like crystals, 30 to 70 nm in diameter, and irregularly disseminated areas.. Read More»
  DOI: 10.4172/2157-7099.1000214

  Abstract PDF