Journal of Bioprocessing & Biotechniques

Otero MC

Publications

• Research Article
  Microencapsulation of Bovine Vaginal Lactobacilli in Alginate Using Emulsion-gelation: Freeze-drying, Storage and Antimicrobial Activity
  Author(s): Ale CE, Torres Luque A, Gonzalez Moreno C and Otero MCAle CE, Torres Luque A, Gonzalez Moreno C and Otero MC
  
  Probiotics containing autochthonous Lactic Acid Bacteria (LAB) as local treatment in the Bovine Reproductive Tract (BRT) was proposed as a sustainable alternative to prevent outcome of pathogens colonization in the postpartum uterus in cows. Microencapsulation of these LABs could improve their survival during stressing conditions and promote the intimate contact between the veterinarian form and the vaginal mucosa. In this work, emulsion-ionic gelation technique was applied to encapsulate bovine LAB strains in an alginate (3%) matrix. Optical and scanning electron microscopic evaluations showed spheroidal particles (12-48 μm) with a fully charge of LAB; the average load ranged 8.98 ± 0.15 to 8.06 ± 0.21 log CFU/g. The microencapsulated lactic acid bacteria (LAB-MCs) stability was evaluated during lyophilization [in Skim Milk (SM), or Neutral.. Read More»
  DOI: 10.4172/2155-9821.1000262

  Abstract PDF